AMB Express

Table 2 Results of CCRD used to assess the influence of temperature, time of induction and IPTG concentration on biomass and soluble ΔNS1 production

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Run	Independent variables^a			Responses
	Independent variables^a			Biomass (DCW g/L)		ΔNS1 (mg/L)
	Temp	Time	IPTG	Experimental	Predicted	Experimental	Predicted
1	− 1.68	0	0	2.02	2.30	229	278
2	+ 1	+ 1	− 1	2.81	3.45	52	7
3	0	− 1.68	0	3.33	3.51	344	279
4	− 1	− 1	− 1	2.10	1.92	168	144
5	+ 1.68	0	0	2.51	1.89	5	14
6	+ 1	+ 1	+ 1	3.17	3.45	97	38
7	+ 1	− 1	+ 1	3.92	4.08	292	286
8	− 1	− 1	+ 1	2.17	1.92	131	175
9	0	0	+ 1.68	4.97	5.16	357	386
10	0	0	0	6.78	7.35	802	969
11	− 1	+ 1	+ 1	6.39	6.09	526	463
12	0	0	− 1.68	5.68	5.16	304	334
13	0	0	0	7.65	7.35	1021	969
14	0	0	0	7.10	7.35	837	969
15	0	+ 1.68	0	6.99	6.48	190	313
16	− 1	+ 1	− 1	5.63	6.09	506	432
17	+ 1	− 1	− 1	3.92	4.08	194	254
18	0	0	0	7.76	7.35	1154	969
19	0	0	0	7.10	7.35	917	969
20	0	0	0	7.65	7.35	1095	969

^aCode values for independent variables, the actual values in Table 1

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