Skip to main content

Table 2 Results of CCRD used to assess the influence of temperature, time of induction and IPTG concentration on biomass and soluble ΔNS1 production

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

RunIndependent variablesaResponses
Biomass (DCW g/L)ΔNS1 (mg/L)
TempTimeIPTGExperimentalPredictedExperimentalPredicted
1− 1.68002.022.30229278
2+ 1+ 1− 12.813.45527
30− 1.6803.333.51344279
4− 1− 1− 12.101.92168144
5+ 1.68002.511.89514
6+ 1+ 1+ 13.173.459738
7+ 1− 1+ 13.924.08292286
8− 1− 1+ 12.171.92131175
900+ 1.684.975.16357386
100006.787.35802969
11− 1+ 1+ 16.396.09526463
1200− 1.685.685.16304334
130007.657.351021969
140007.107.35837969
150+ 1.6806.996.48190313
16− 1+ 1− 15.636.09506432
17+ 1− 1− 13.924.08194254
180007.767.351154969
190007.107.35917969
200007.657.351095969
  1. aCode values for independent variables, the actual values in Table 1