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Table 1 Independent variables and levels of the experimental design

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Independent variable

Level

− αa

− 1

0

+ 1

+ αa

A: Temperature (°C)

11.2

16

23

30

34.8

B: Time (h)

7.9

12

18

24

28.1

C: IPTG (mM)

0.2

0.4

0.7

1.0

1.2

  1. a α = 1.68