From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology
Independent variable
Level
− αa
− 1
0
+ 1
+ αa
A: Temperature (°C)
11.2
16
23
30
34.8
B: Time (h)
7.9
12
18
24
28.1
C: IPTG (mM)
0.2
0.4
0.7
1.0
1.2