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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Fig. 4

Antigenicity and specificity of the recombinant protein ΔNS1. a Coomassie Blue staining (left panels) and Western blots (right panels) obtained with 1 µg of purified ΔNS1 or full length Brazilian ZIKV NS1 and DENV2 NS1 (strain NGC). Western blots were probed with a mAb anti-His-Tag (ZIKV proteins) or anti-DENV2-NS1. b Reaction of human immune sera from eight DENV-infected subjects (serotypes 1 to 4) with DENV NS1, ZIKV NS1 and ΔNS1 in ELISA. c Reaction of ZIKV+ DENV+ human serum sample with intact and heat denatured DENV NS1, ZIKV NS1 and ΔNS1. Statistical significance was assessed by two-way ANOVA and the Bonferroni test

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