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Fig. 3 | AMB Express

Fig. 3

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Fig. 3

Solubility of ΔNS1 during the induction in the bioreactor. After inoculation to achieve OD 0.1, culture was maintained at 37 °C until OD ~ 2.0 (pre-induction). Temperature was shifted to 21 °C and protein production induced with 0.7 mM IPTG. To determine solubility and biomass concentration, aliquots were taken at regular intervals after inoculation. After lysis, soluble and insoluble protein extracts were separated by SDS-PAGE and stained by Coomassie Blue. NI = non-induced. OD converted to biomass (g/L) according to the relation 1.0 OD = 0.34 g/L

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