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Fig. 1 | AMB Express

Fig. 1

From: Optimization and scale-up production of Zika virus ΔNS1 in Escherichia coli: application of Response Surface Methodology

Fig. 1

Response surface plots for biomass and production of soluble ΔNS1 as a function of time, temperature of induction and IPTG concentration. The model generated by the CCD for ac biomass concentration measured as dry cell weight (DCW g/L) and df production of ΔNS1 the soluble fraction of cell extracts (mg/L). The temperature range was 16–30 °C, time between 12 and 24 h of induction and IPTG concentration between 0.4 and 1.0 mM. Color-coding indicates high (red) and low (blue) responses

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