Skip to main content

Table 1 LC and MS conditions used in AHL detection

From: Effect of the luxI/R gene on AHL-signaling molecules and QS regulatory mechanism in Hafnia alvei H4

LC condition LC condition ϕ4.6 × 150 mm, 5 µm (Agilent)
Column temperature 30 °C
Inject volume 6 μL
Mobile phase Water/acetonitrile
Flow rate 1.0 mL/min
Measurement time 35 min
MS condition MS 5500 AB SCIEX
Ionization mode ESI+
Ion source Turbo spray
Curtain gas (CUR) 20 psi
Ionspray voltage (IS) 5000 V
Temperature (TEM) 450 °C
Ion source gas1 (GS1) 30 psi
Ion source gas2 (GS2) 10 psi
Collision gas (CAD) Medium
  1. AHL acyl homoserine lactone, LCMS liquid chromatography–tandem mass spectrometry