Skip to main content

Table 1 LC and MS conditions used in AHL detection

From: Effect of the luxI/R gene on AHL-signaling molecules and QS regulatory mechanism in Hafnia alvei H4

LC conditionLC conditionϕ4.6 × 150 mm, 5 µm (Agilent)
Column temperature30 °C
Inject volume6 μL
Mobile phaseWater/acetonitrile
Flow rate1.0 mL/min
Measurement time35 min
MS conditionMS5500 AB SCIEX
Ionization modeESI+
Ion sourceTurbo spray
Curtain gas (CUR)20 psi
Ionspray voltage (IS)5000 V
Temperature (TEM)450 °C
Ion source gas1 (GS1)30 psi
Ion source gas2 (GS2)10 psi
Collision gas (CAD)Medium
  1. AHL acyl homoserine lactone, LCMS liquid chromatography–tandem mass spectrometry