Compound no | Colour | λmax in visible (in nm) | m/z [M+H]+ (in g mol−1) | Extraction solvent (amount of the metabolites in each extract) | Total amount extracted (in meqv L−1 of culture broth) | |||||
---|---|---|---|---|---|---|---|---|---|---|
H2O | 50% MeOH | 50% EtOH | MeOH | MeOH/EtOH | EtOH | |||||
Solvent polarity index: | Â | Â | Â | 10.0 | 7.5 | 7.0 | 5.0 | 4.5 | 4.0 | |
Mycelium grown in DMD | ||||||||||
 Bikaverin 1 | Red | 507 | 383 | 0.5 ± 0.1 | 3.7 ± 0.2 | 4.8 ± 0.4 | 7.1 ± 0.4 | 10.3 ± 0.8a | 4.8 ± 0.3 | 31.2 ± 2.2 |
 Norbikaverin 2 | Red | 509 | 369 | < 0.1 | 11.2 ± 0.8 | 20.4 ± 1.2b | 13.0 ± 0.9 | 12.1 ± 0.6 | 4.8 ± 0.2 | 61.5 ± 3.7 |
 Oxo-pre-bikaverin 3 | Yellow | 382, 441 | 339 | < 0.1 | 2.7 ± 0.2c | 2.1 ± 0.2 | 1.6 ± 0.2 | 1.1 ± 0.1 | 0.2 ± 0.1 | 7.7 ± 0.8 |
 Me-oxo-pre-bikaverin 4 | Yellow | 376, 440 | 353 | < 0.1 | 3.8 ± 0.3d | 3.6 ± 0.2 | 2.7 ± 0.3 | 1.7 ± 0.2 | 0.9 ± 0.2 | 12.6 ± 1.2 |
 Dinor-bikaverin 5 | Yellow | 457 | 357 | < 0.1 | 0.7 ± 0.2 | < 0.1 | < 0.1 | < 0.1 | < 0.1 | 0.7 ± 0.2 |
 Pre-bikaverin 6 | Yellow | 438 | 323 | < 0.1 | < 0.1 | 0.2 ± 0.1 | < 0.1 | < 0.1 | < 0.1 | 0.2 ± 0.1 |
 Pigment ni 7a | Yellow | 376, 437 | nd | nd | nd | nd | nd | nd | nd | nd |
 Pigment ni 7b | Purple | 496, 523 | nd | < 0.1 | 0.4 ± 0.1 | 0.5 ± 0.1 | < 0.1 | < 0.1 | < 0.1 | 1.0 ± 0.2 |
 Pigment ni 7c | Purple | 491, 530 | nd | < 0.1 | < 0.1 | < 0.1 | 0.7 ± 0.2 | 0.5 ± 0.1 | 0.3 ± 0.1 | 1.5 ± 0.4 |
 Pigment ni 8 | Yellow | 430 | nd | < 0.1 | 0.4 ± 0.1 | 0.7 ± 0.2 | 0.8 ± 0.1 | 0.7 ± 0.1 | 0.7 ± 0.2 | 3.3 ± 0.7 |
 Pigment ni 9 | Purple | 516, 549 | nd | < 0.1 | 1.9 ± 0.2 | 1.3 ± 0.3 | 0.2 ± 0.1 | < 0.1 | < 0.1 | 3.4 ± 0.5 |
 Total pigments extracted (in meqv L−1 culture broth) |  |  |  | 0.5 ± 0.3 | 24.8e ± 2.1 | 33.6f ± 2.7 | 26.2g ± 2.2 | 26.4h ± 1.9 | 11.6 ± 1.1 | 123.1 ± 10.3 |
 > Other metabolites extracted | ||||||||||
  Beauvericin |  | – | 784 | nd | nd | nd | nd | nd | nd | nd |
  Ergosterol derivate |  | – | 393 | < 0.1 | < 0.1 | 0.9 ± 0.1 | 12.8 ± 1.2 | 7.3 ± 0.4 | 0.5 ± 0.1 | 21.6 ± 1.8 |
  Others (ni) |  | – | nd | 5.8 ± 0.4 | 12.8 ± 0.8 | 0.3 ± 0.1 | 0.5 ± 0.1 | 0.6 ± 0.2 | 1.3 ± 0.3 | 21.3 ± 1.9 |
Mycelium grown in PDB | ||||||||||
 Bikaverin 1 | Red | 507 | 383 | 1.0 ± 0.3 | 1.2 ± 0.2 | 2.3 ± 0.2 | 2.8 ± 0.5 | 7.8 ± 0.7a | 2.3 ± 0.3 | 17.6 ± 2.1 |
 Norbikaverin 2 | Red | 509 | 369 | 0.5 ± 0.1 | 2.3 ± 0.3 | 5.6 ± 0.4b | 3.4 ± 0.3 | 2.7 ± 0.2 | 0.6 ± 0.1 | 15.1 ± 1.4 |
 Oxo-pre-bikaverin 3 | Yellow | 382, 441 | 339 | < 0.1 | 7.1 ± 0.4c | 3.6 ± 0.3 | 1.7 ± 0.4 | 0.6 ± 0.2 | < 0.1 | 13.0 ± 1.3 |
 Me-oxo-pre-bikaverin 4 | Yellow | 376, 440 | 353 | < 0.1 | 8.3 ± 0.4d | 3.7 ± 0.2 | 2.2 ± 0.2 | 1.0 ± 0.3 | < 0.1 | 15.3 ± 1.1 |
 Dinor-bikaverin 5 | Yellow | 457 | 357 | < 0.1 | 0.6 ± 0.1 | 0.3 ± 0.1 | 0.1 ± 0.1 | < 0.1 | < 0.1 | 1.1 ± 0.3 |
 Pre-bikaverin 6 | Yellow | 438 | 323 | < 0.1 | < 0.1 | 0.2 ± 0.2 | < 0.1 | < 0.1 | < 0.1 | 0.3 ± 0.2 |
 Pigment ni 7a | Yellow | 376, 437 | nd | < 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 | 0.5 ± 0.2 | 0.5 ± 0.2 | < 0.1 | 1.5 ± 0.6 |
 Pigment ni 7b | Purple | 496, 523 | nd | nd | nd | nd | nd | nd | nd | nd |
 Pigment ni 7c | Purple | 491, 530 | nd | nd | nd | nd | nd | nd | nd | nd |
 Pigment ni 8 | Yellow | 430 | nd | < 0.1 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 | 0.4 ± 0.1 | < 0.1 | 1.1 ± 0.4 |
 Pigment ni 9 | Purple | 516, 549 | nd | nd | nd | nd | nd | nd | nd | nd |
 Total pigments extracted (in meqv L−1 culture broth) |  |  |  | 1.5 ± 0.3 | 20.1i ± 1.6 | 16.0j ± 1.6 | 11.2k ± 1.8 | 13.2l ± 1.7 | 3.0 ± 0.4 | 65.0 ± 7.4 |
 > Other metabolites extracted | ||||||||||
  Beauvericin |  | – | 784 | nd | nd | nd | nd | nd | nd | nd |
  Ergosterol derivate |  | – | 393 | < 0.1 | < 0.1 | 0.4 ± 0.2 | 5.0 ± 0.4 | 4.1 ± 0.3 | 0.7 ± 0.2 | 10.3 ± 1.1 |
  Others (ni) |  | – | nd | 48.2 ± 2.9 | 5.6 ± 0.3 | 2.1 ± 0.3 | 0.3 ± 0.2 | 0.4 ± 0.2 | 0.9 ± 0.3 | 57.5 ± 4.2 |