Table 2 Plasmids and Salmonella typhimurium strains used in this study
From: Secreting-lux/pT-ClyA engineered bacteria suppresses tumor growth via interleukin-1β in two pathways
Strains/plasmids | Description |
|---|---|
pGEM-T vector | Purchased from Promega |
pJL30A | pGEM-T vector encode into the “tetRA” of PCR product |
pJL32 | Deleted TetA gene of pJL30A and encode “RBS-MCS I” of PCR product under the TetA promoter |
pJL37 | Encode the ‘RBS-MCS II’ of PCR product into pGEM-T vector |
pJL39 | pJL32 and pJL37 were digested by Nru I and Sca I restriction enzymes, the construction of pJL39 was ligated by the small fragment of pJL32 and large fragment of pJL37 |
pJL43 (pT-ClyA) ΔppGpp Salmonella typhimurium | pJL39 encode into cytolysin A under TetA promoter Salmonella typhimurium is defective in ppGpp |
S.t-ΔpGlux | ΔppGpp Salmonella typhimurium with the luciferase gene operon luxCDABE |
S.t-ΔpGlux/pT-ClyA | pJL43 was transformed into S.t-ΔpGlux |