Table 1 Primers used in this study
From: Secreting-lux/pT-ClyA engineered bacteria suppresses tumor growth via interleukin-1β in two pathways
Target | Primer | Template |
|---|---|---|
Tetra | RItetR, 5′-TTAAGACCCACTTTCACATT-3′ | TH9952 |
RItetA, 5′-CTAAGCACTTGTCTCCTG-3′ | ||
RBS-MCS I | TetPXbaIF, 5′-ACTTTTATCTAATCTAGACATCA-3′ | pJL30A |
TetPXbaIrev, 5′-GCCGCCATGGCCCGGGATCCTGCAGGCCTTCTCTATCACTGATAGGGAGT-3′ | ||
RBS-MCS II | TetRNruIF, 5′-TACTAAGTCATCGCGATGGAGCAA-3′ | pJL30A |
TerRNruIrev, 5′-AATCCTCGACAGGCTTCTCGAGTGGCCACTCCTGCTTAAGACCCACTTTCACATTTAAGT-3′ | ||
ClyA(A) | ClyAtetAProF, 5′-GTGAAATGACCGGAATATTTGCAGAACAAACT-3′ | pAClyA |
ClyAtetAProRev, 5′-ACGCGGATCCTCAGACGTCAGGAACCTCGAA-3′ |