AMB Express

Table 1 Number of bacteria (log CFU mL⁻¹) and antibacterial activity (A) of QAS- and AgNPs-functionalized membranes on supporting layers (MFRF, VS, PP) and their respective non-functionalized control membranes

From: Resazurin assay for assessment of antimicrobial properties of electrospun nanofiber filtration membranes

	E. coli				E. faecalis				WWTP effluent
	Resazurin assay		Plate count assay		Resazurin assay		Plate count assay		Resazurin assay
	log CFU mL⁻¹ ± SD	A	log CFU mL⁻¹	A	log CFU mL⁻¹ ± SD	A	log CFU mL⁻¹	A	log CFU_EEQ mL⁻¹ ± SD	A
QAS-MFRF	8.06 ± 0.55	0.01	8.02	0.76	< LOQ	< 1	5.3	0.54	4.60 ± 1.13	0.31
PU-MFRF	8.07 ± 0.36	0.01	8.67	0.76	< LOQ	< 1	5.22	0.54	4.91 ± 0.71	0.31
QAS-VS	9.13 ± 0.80	− 0.35	8.52	− 0.08	7.11 ± 0.63	0.25	7.09	0.36	6.49 ± 0.25	0.40
PU-VS	8.78 ± 0.64	− 0.35	8.44	− 0.08	7.36 ± 0.35	0.25	7.45	0.36	6.79 ± 0.22	0.40
QAS-PP	9.68 ± 0.41	0.02	8.48	0.03	7.94 ± 0.06	0.02	7.71	0.49	7.63 ± 0.46	0.11
PU-PP	9.70 ± 0.27	0.02	8.51	0.03	7.96 ± 0.11	0.02	8.19	0.49	7.74 ± 0.44	0.11
AgNPs-PP	< LOQ	> 6.70	3.27	5.24	< LOQ	> 4.96	3.45	4.63	< LOQ	> 4.74
PU-PP	9.70 ± 0.27	> 6.70	8.51	5.24	7.96 ± 0.11	> 4.96	8.19	4.63	7.74 ± 0.44	> 4.74
Inoculum w/o membr.	9.95 ± 0.02	–	–	–	7.98 ± 0.25	–	–	–	7.72 ± 0.32	–

A was determined either by resazurin microplate assay or the plate count assay. A was calculated from Eq. 1 for resazurin assay and Eq. 2 for plate count assay and expressed as log CFU reduction compared to respective non-functionalized control membrane. A number of bacteria in the WWTP effluent sample was calculated from the E. coli calibration curve, therefore membrane A against WWTP effluent microbial community was expressed as a reduction of log CFU_EEQ mL⁻¹

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