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Fig. 5 | AMB Express

Fig. 5

From: Development of a multiplex PCR assay for the simultaneous and rapid detection of six pathogenic bacteria in poultry

Fig. 5

Determination of the sensitivity of the multiplex PCR for bacterial genomic DNA detection. Lane M: 2000 bp DNA marker; a Lanes 1–10: the concentration of Escherichia coli DNA were 100 ng, 75 ng, 50 ng, 25 ng, 12.5 ng, 7.5 ng, 2.5 ng, 1 ng, 500 pg and 250 pg, respectively. b Lanes 1–10: the concentration of Pasteurella multocida DNA were 100 ng, 75 ng, 50 ng, 25 ng, 12.5 ng, 7.5 ng, 2.5 ng, 1 ng, 500 pg and 250 pg, respectively. c Lanes 1-8: the concentration of Proteus mirabilis DNA were 100 ng, 75 ng, 50 ng, 25 ng, 12.5 ng, 7.5 ng, 2.5 ng, 1 ng, 500 pg and 250 pg, respectively. d Lanes 1–10: the concentration of Pseudomonas aeruginosa DNA were 100 ng, 75 ng, 50 ng, 25 ng, 12.5 ng, 7.5 ng, 2.5 ng, 1 ng, 500 pg and 250 pg, respectively. e Lanes 1–10: the concentration of Salmonella spp. DNA were 100 ng, 75 ng, 50 ng, 25 ng, 12.5 ng, 7.5 ng, 2.5 ng, 1 ng, 500 pg and 250 pg, respectively. f Lanes 1–10: the concentration of Staphylococcus aureus DNA concentration were 100 ng, 75 ng, 50 ng, 25 ng, 12.5 ng, 7.5 ng, 2.5 ng, 1 ng, 500 pg and 250 pg, respectively

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