Fig. 4

Determination of specificity of the multiplex PCR. Lane M: 2000 bp DNA marker; a Lane 1: the template of m-PCR contain 6 bacterial genomes as positive control. Lane 2: the template of m-PCR was Klebsiella pneumoniae; Lane 3: the template of m-PCR was Shigella spp.; Lane 4: the template of m-PCR was Bacillus subtilis; Lane 5: the template of m-PCR was Bacillus cereus; Lane 6: the template of m-PCR was Enterococcus faecalis; Lane 7: the template of m-PCR was Listeria monocytogenes; Lane 8: the template of m-PCR was Streptococcus suis; Lane 9: the template of m-PCR was Cryptosporidium baileyi; Lane 10: the template of m-PCR was Eimeria tenella; Lane 11: the template of m-PCR was Newcastle disease virus (NDV); Lane 12: the template of m-PCR was Avian Influenza virus H9N2; Lane 13: the template of m-PCR was infectious bursal disease virus (IBDV); Lane 14: Negative control. b Lanes 1–3: the template of m-PCR was O₁, O₂ and O₇₈ serotype of avian pathogenic Escherichia coli, respectively; Lanes 5–7: the template of m-PCR was Salmonella typhimurium, Salmonella enteritidis, Salmonella pullorum, respectively; Lanes 4, 8: negative control