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Table 2 Primer characteristics and parameters evaluated by qPCR

From: Two-step genomic sequence comparison strategy to design Trichoderma strain-specific primers for quantitative PCR

CodeOptimum conditionsR2SlopeEfficiency (%)
P61 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s0.9986− 3.2383103.61
P71 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s0.9967− 3.2561102.82
P81 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 60 °C for 34 s0.9989− 3.346698.98
P101 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 60 °C for 34 s0.9994− 3.370498.02
P111 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s0.9958− 3.2657102.40
P121 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s0.9973− 3.251103.05
P131 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s0.9971− 3.403896.69
ITS11 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 58 °C for 34 s0.9834− 3.1986105.42