Two-step genomic sequence comparison strategy to design Trichoderma strain-specific primers for quantitative PCR

AMB Express

Table 2 Primer characteristics and parameters evaluated by qPCR

Code	Optimum conditions	R²	Slope	Efficiency (%)
P6	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s	0.9986	− 3.2383	103.61
P7	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s	0.9967	− 3.2561	102.82
P8	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 60 °C for 34 s	0.9989	− 3.3466	98.98
P10	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 60 °C for 34 s	0.9994	− 3.3704	98.02
P11	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s	0.9958	− 3.2657	102.40
P12	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s	0.9973	− 3.251	103.05
P13	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 62 °C for 34 s	0.9971	− 3.4038	96.69
ITS1	1 min incubation at 95 °C, 40 cycles consisting of 95 °C for 15 s and 58 °C for 34 s	0.9834	− 3.1986	105.42