Molecular characterization of hypothetical scaffolding-like protein S1 in multienzyme complex produced by Paenibacillus curdlanolyticus B-6

AMB Express

Table 2 Binding affinities and capacities of rS1 and its truncated derivatives toward the peptidoglycan cell surface layer and insoluble substrates

Substrates	K_d (µM)^a			[PC]max (µmol/g substrate)^a
Substrates	rS1	rS1ΔSLH	rSLH	rS1	rS1ΔSLH	rSLH
Peptidoglycan layer	0.10 ± 0.05	ND^b	0.12 ± 0.04	0.19 ± 0.1	ND	0.15 ± 0.1
Microcrystalline cellulose	0.23 ± 0.05	ND	0.27 ± 0.05	0.27 ± 0.1	ND	0.23 ± 0.1
Insoluble xylan	0.22 ± 0.04	1.32 ± 0.2	0.32 ± 0.03	0.24 ± 0.1	0.20 ± 0.04	0.20 ± 0.1

Data are presented as the means from three independent experiments. Error bars represent the standard deviation (n = 3)
^aThe binding assay was performed in triplicate, and the K_d and [PC]max values were calculated by using a double-reciprocal plot
^bND, no binding detected