Table 1 Enzymatic characterization of nS1, rS1 and rXyn11A
Property | nS1 | rS1 | rXyn11A |
|---|---|---|---|
Molecular mass (kDa) | 280 | 91 | 40 |
Structure | SLH-unknown glycoprotein | SLH-unknown | GH11-linker-CBM36 |
Temperature optimum (stability) | 60 °C (< 60 °C) | 60 °C (< 60 °C) | 60 °C |
pH optimum (stability) | 6.0 (6.0–8.0) | 6.0 (6.0–8.0) | 6.0 (6.0–7.0) |
Km (mg/ml) | 29.5 ± 2.0 | 19.5 ± 3.0 | 1.5 ± 0.1 |
Kcat (1/s) | 66.1 ± 0.1 | 162.2 ± 2.0 | 204.4 ± 3.0 |