Fig. 4

Binding properties of rS1 and its truncated derivatives. Binding to microcrystalline cellulose (a), other polysaccharides, such as insoluble xylan and chitin (b), and the bacterial peptidoglycan layer (c), as revealed by SDS-PAGE. Each recombinant protein was incubated with microcrystalline cellulose, another polysaccharide or the peptidoglycan layer for 4 h at 30 °C. Unbound (lane U) and bound (lane B) protein fractions were separated by centrifugation; pellets were washed three times (lane W). b Lane 1, binding fraction of microcrystalline cellulose; lane 2, binding fraction of acid swollen cellulose; lane 3, binding fraction of insoluble birchwood xylan; lane 4, binding fraction of arabinan; lane 5, binding fraction of chitin. Lane M, molecular mass markers (kDa). Lanes U, W, and B of rS1 (a) are from an SDS-PAGE gel. Lanes U, W and B of rS1ΔSLH and rSLH were cropped from different SDS-PAGE gels, respectively