Fig. 1
From: Identification of novel B cell epitopes in the fiber protein of serotype 8 Fowl adenovirus
Identification of two mAbs 5F10 and 4D9 against fiber protein of FAdV-8. LMH cells infected with 0.01 MOI of FAdV-8 or transfected with pcDNA3.1-F were analyzed using mAbs by IFA and western blot. a The LMH cells infected with FAdV-8 or transfected with pcDNA3.1-F were analyzed using mAbs by IFA. The uninfected LMH cells and the LMH cells transfected with pcDNA3.1 were as negative control. b The lysates of LMH cells infected with FAdV-8 were analyzed using mAbs by western blot. Lanes 1 and 2: lysates of LMH cells infected without or with FAdV-8, respectively. c The lysates of LMH cells transfected with pcDNA3.1-F were analyzed using mAbs by western blot. Lanes 1 and 2: lysates of LMH cells transfected with pcDNA3.1 and pcDNA3.1-F, respectively