Using submerged fermentation to fast increase N6-(2-hydroxyethyl)-adenosine, adenosine and polysaccharide productions of Cordyceps cicadae NTTU 868

AMB Express

Table 4 Effect of different concentration of dextrose, yeast extract and KCl on adenosine, HEA and polysaccharide in 100-mL submerged-fermented C. cicadae NTTU868 mycelium

Sources	Adenosine (mg/kg biomass)	HEA (mg/kg biomass)	Polysaccharide (g/kg biomass)
Extra dextrose (%)
0.00	1080 ± 56^b	533 ± 33^c	20.5 ± 1.1^b
0.04	1020 ± 56^b	472 ± 15^bc	18.9 ± 2.0^ab
0.20	964 ± 90^b	432 ± 59^b	17.6 ± 0.2^a
1.00	656 ± 125^b	337 ± 24^a	19.4 ± 0.9^ab
Extra yeast extract (%)
0.00	1080 ± 56^a	533 ± 33^a	20.5 ± 1.1^a
0.04	1134 ± 81^a	544 ± 38^a	21.3 ± 4.0^a
0.20	1157 ± 376^a	764 ± 120^b	20.8 ± 2.5^a
1.00	1169 ± 22^a	634 ± 137^ab	22.4 ± 6.1^a
Extra KCl (%)
0.00	1080 ± 56^c	533 ± 33^b	20.5 ± 1.1^a
0.01	1035 ± 60^c	479 ± 40^b	21.2 ± 1.0^a
0.05	841 ± 38^b	474 ± 81^b	20.0 ± 1.6^a
0.25	670 ± 56^a	356 ± 51^a	21.6 ± 0.7^a

The data is presented as the mean ± SD (n = 3)
KCl potassium chloride
^a,b,cDifferent letters indicate significantly different values according to a one-way ANOVA with Duncan’s multiple test (p < 0.05)