Fig. 1

Stability of N-PfCelTOS and CH-PfCelTOS incubated at 37 °C and 65 °C. a Coomassie blue total protein staining of N- and CH-proteins incubated at A: 37 °C and B: 65 °C for 1, 4 and up to 24 h. b Western blot analysis using anti-histidine mAb and c Western blot analysis using PfCelTOS-C-term specific mAb 3D11.D4. Lane 1 for each gel or blot is the reference PfCelTOS protein, untreated. d Lipid strip analysis of N- and CH-PfCelTOS incubated at 37 °C for up to 24 h. T0 indicates control lipid strips for assessing changes in binding profile of both the proteins