Fig. 2
From: Towards the understanding of the enzymatic cleavage of polyisoprene by the dihaem-dioxygenase RoxA
EPR-spectra of RoxA-F317Y as isolated compared to RoxA-Wt. a The difference spectrum (blue) of F317Y (red) minus the RoxA-Wt-spectrum (black) displays additional EPR-intensity representing a characteristic haem–O–Tyr coordinated low-spin species. b EPR-spectra of RoxA-F317Y as isolated (red, different preparation as in a) and after incubation with pyridine (5 mM, 15 min at 4 °C) (green) resulting in almost total replacement of the Tyr-ligation by pyridine