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Table 2 Overview of different microbial strains engineered to produce isobutanol (specific growth rates and isobutanol titer) including those that were generated in this study

From: Systematic improvement of isobutanol production from d-xylose in engineered Saccharomyces cerevisiae

Strain name

Genotype

Description

Specific growth rate in glucose (h−1)

Specific growth rate in xylose (h−1)

Isobutanol titer in glucose (m/L)a

Isobutanol titer in xylose (m/L)a

Isobutanol productivity in xylose (mg/gCDW−1h−1)

References

Isoy16

ilv2Δ0; ilv5Δ0; ilv3Δ0 p425-synthILV235, pRS42HH7-Aro10-Ash2, pRS42NH7-Tal1-Xks1, pRS24H7-synthXI

CEN.PK with deletions in Ilv2, Ilv5 and Ilv3 overexpressing XylA from C. phytofermentans, XKS1 and Aro10 from S. cerevisiae and cytosol-targeted Ilv2, Ilv5 and Ilv3 from S. cerevisiae

Not reported

Not reported

Not reported

1.36 ± 0.11b

Not reported

Brat and Boles (2013)

BY4742

Mat α; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0

None

0.1395 ± 0.0003

N.D.

4.0 ± 0.5

N.D.

N.D.

This study

PWY0013

BY4742 ARS208::PTEF1-SsXKS; YMRWΔ15::PTEF1-HXTF79S

BY4742 overexpressing XKS from Scheffersomyces stipitis, HXT7_F79S from Saccharomyces cerevisiae

0.1329 ± 0.0001

N.D.

5.5 ± 0.7

N.D.

N.D.

This study

PWY1113

PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-CtXYL2

PWY0013 overexpressing XYL1 and XYL2 from Candida tropicalis

0.1435 ± 0.0004

0.0313 ± 0.0011

2.8 ± 0.1

3.8 ± 0.4

0.047 ± 0.005

This study

PWY2313

PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-SsXYL2

PWY0013 overexpressing XYL1 and XYL2 from S. stipitis

0.1461 ± 0.0003

0.0423 ± 0.0004

5.0 ± 0.4

4.3 ± 0.7

0.052 ± 0.008

This study

PWY1123

PWY1113 GRE3Δ

PWY1113 with GRE3 deleted

0.1472 ± 0.0003

0.0295 ± 0.0012

13.9 ± 0.9

5.7 ± 0.6

0.076 ± 0.008

This study

PWY2323

PWY2313 GRE3Δ

PWY2313 with GRE3 deleted

0.1436 ± 0.0002

0.0426 ± 0.0001

23.5 ± 0.7

4.4 ± 0.4

0.053 ± 0.005

This study

PWY1143

PWY1123 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3; YORW Δ22::PTDH3 –LlkivDmt-T2A-ScADH7mt

PWY1123 overexpressing Ilv2, Ilv5, Ilv3, ADH7 from S. cerevisiae and kivD from L. lactis

0.1463 ± 0.0003

0.0368 ± 0.0015

30.4 ± 1.0

9.4 ± 0.3

0.114 ± 0.006

This study

PWY2343

PWY2323 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3; YORW Δ22::PTDH3 –LlkivDmt-T2A-ScADH7mt

PWY2323 overexpressing Ilv2, Ilv5, Ilv3, ADH7 from S. cerevisiae and kivD from L. lactis

0.1476 ± 0.0004

0.0431 ± 0.0004

43.3 ± 1.0

16.9 ± 1.9

0.226 ± 0.014

This study

PWY1153

PWY1143 PHO13Δ

PWY1143 with PHO13 deleted

0.1408 ± 0.0003

0.0513 ± 0.0002

33.9 ± 1.2

11.8 ± 0.8

0.134 ± 0.010

This study

PWY2353

PWY2343 PHO13Δ

PWY2343 with PHO13 deleted

0.1469 ± 0.0003

0.0532 ± 0.0004

43.0 ± 0.9

19.7 ± 2.4

0.172 ± 0.016

This study

  1. N.D. represents not detected due to strain’s inability to grow
  2. aIsobutanol titers after 2 days of cultivation in yeast selective medium
  3. bIsobutanol titers after 6 days of cultivation in yeast selective medium