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Table 2 Overview of different microbial strains engineered to produce isobutanol (specific growth rates and isobutanol titer) including those that were generated in this study

From: Systematic improvement of isobutanol production from d-xylose in engineered Saccharomyces cerevisiae

Strain name Genotype Description Specific growth rate in glucose (h−1) Specific growth rate in xylose (h−1) Isobutanol titer in glucose (m/L)a Isobutanol titer in xylose (m/L)a Isobutanol productivity in xylose (mg/gCDW−1h−1) References
Isoy16 ilv2Δ0; ilv5Δ0; ilv3Δ0 p425-synthILV235, pRS42HH7-Aro10-Ash2, pRS42NH7-Tal1-Xks1, pRS24H7-synthXI CEN.PK with deletions in Ilv2, Ilv5 and Ilv3 overexpressing XylA from C. phytofermentans, XKS1 and Aro10 from S. cerevisiae and cytosol-targeted Ilv2, Ilv5 and Ilv3 from S. cerevisiae Not reported Not reported Not reported 1.36 ± 0.11b Not reported Brat and Boles (2013)
BY4742 Mat α; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0 None 0.1395 ± 0.0003 N.D. 4.0 ± 0.5 N.D. N.D. This study
PWY0013 BY4742 ARS208::PTEF1-SsXKS; YMRWΔ15::PTEF1-HXTF79S BY4742 overexpressing XKS from Scheffersomyces stipitis, HXT7_F79S from Saccharomyces cerevisiae 0.1329 ± 0.0001 N.D. 5.5 ± 0.7 N.D. N.D. This study
PWY1113 PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-CtXYL2 PWY0013 overexpressing XYL1 and XYL2 from Candida tropicalis 0.1435 ± 0.0004 0.0313 ± 0.0011 2.8 ± 0.1 3.8 ± 0.4 0.047 ± 0.005 This study
PWY2313 PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-SsXYL2 PWY0013 overexpressing XYL1 and XYL2 from S. stipitis 0.1461 ± 0.0003 0.0423 ± 0.0004 5.0 ± 0.4 4.3 ± 0.7 0.052 ± 0.008 This study
PWY1123 PWY1113 GRE3Δ PWY1113 with GRE3 deleted 0.1472 ± 0.0003 0.0295 ± 0.0012 13.9 ± 0.9 5.7 ± 0.6 0.076 ± 0.008 This study
PWY2323 PWY2313 GRE3Δ PWY2313 with GRE3 deleted 0.1436 ± 0.0002 0.0426 ± 0.0001 23.5 ± 0.7 4.4 ± 0.4 0.053 ± 0.005 This study
PWY1143 PWY1123 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3; YORW Δ22::PTDH3LlkivDmt-T2A-ScADH7mt PWY1123 overexpressing Ilv2, Ilv5, Ilv3, ADH7 from S. cerevisiae and kivD from L. lactis 0.1463 ± 0.0003 0.0368 ± 0.0015 30.4 ± 1.0 9.4 ± 0.3 0.114 ± 0.006 This study
PWY2343 PWY2323 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3; YORW Δ22::PTDH3LlkivDmt-T2A-ScADH7mt PWY2323 overexpressing Ilv2, Ilv5, Ilv3, ADH7 from S. cerevisiae and kivD from L. lactis 0.1476 ± 0.0004 0.0431 ± 0.0004 43.3 ± 1.0 16.9 ± 1.9 0.226 ± 0.014 This study
PWY1153 PWY1143 PHO13Δ PWY1143 with PHO13 deleted 0.1408 ± 0.0003 0.0513 ± 0.0002 33.9 ± 1.2 11.8 ± 0.8 0.134 ± 0.010 This study
PWY2353 PWY2343 PHO13Δ PWY2343 with PHO13 deleted 0.1469 ± 0.0003 0.0532 ± 0.0004 43.0 ± 0.9 19.7 ± 2.4 0.172 ± 0.016 This study
  1. N.D. represents not detected due to strain’s inability to grow
  2. aIsobutanol titers after 2 days of cultivation in yeast selective medium
  3. bIsobutanol titers after 6 days of cultivation in yeast selective medium