From: Systematic improvement of isobutanol production from d-xylose in engineered Saccharomyces cerevisiae
Strain name | Genotype | Description | Specific growth rate in glucose (h−1) | Specific growth rate in xylose (h−1) | Isobutanol titer in glucose (m/L)a | Isobutanol titer in xylose (m/L)a | Isobutanol productivity in xylose (mg/gCDW−1h−1) | References |
---|---|---|---|---|---|---|---|---|
Isoy16 | ilv2Δ0; ilv5Δ0; ilv3Δ0 p425-synthILV235, pRS42HH7-Aro10-Ash2, pRS42NH7-Tal1-Xks1, pRS24H7-synthXI | CEN.PK with deletions in Ilv2, Ilv5 and Ilv3 overexpressing XylA from C. phytofermentans, XKS1 and Aro10 from S. cerevisiae and cytosol-targeted Ilv2, Ilv5 and Ilv3 from S. cerevisiae | Not reported | Not reported | Not reported | 1.36 ± 0.11b | Not reported | Brat and Boles (2013) |
BY4742 | Mat α; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0 | None | 0.1395 ± 0.0003 | N.D. | 4.0 ± 0.5 | N.D. | N.D. | This study |
PWY0013 | BY4742 ARS208::PTEF1-SsXKS; YMRWΔ15::PTEF1-HXTF79S | BY4742 overexpressing XKS from Scheffersomyces stipitis, HXT7_F79S from Saccharomyces cerevisiae | 0.1329 ± 0.0001 | N.D. | 5.5 ± 0.7 | N.D. | N.D. | This study |
PWY1113 | PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-CtXYL2 | PWY0013 overexpressing XYL1 and XYL2 from Candida tropicalis | 0.1435 ± 0.0004 | 0.0313 ± 0.0011 | 2.8 ± 0.1 | 3.8 ± 0.4 | 0.047 ± 0.005 | This study |
PWY2313 | PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-SsXYL2 | PWY0013 overexpressing XYL1 and XYL2 from S. stipitis | 0.1461 ± 0.0003 | 0.0423 ± 0.0004 | 5.0 ± 0.4 | 4.3 ± 0.7 | 0.052 ± 0.008 | This study |
PWY1123 | PWY1113 GRE3Δ | PWY1113 with GRE3 deleted | 0.1472 ± 0.0003 | 0.0295 ± 0.0012 | 13.9 ± 0.9 | 5.7 ± 0.6 | 0.076 ± 0.008 | This study |
PWY2323 | PWY2313 GRE3Δ | PWY2313 with GRE3 deleted | 0.1436 ± 0.0002 | 0.0426 ± 0.0001 | 23.5 ± 0.7 | 4.4 ± 0.4 | 0.053 ± 0.005 | This study |
PWY1143 | PWY1123 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3; YORW Δ22::PTDH3 –LlkivDmt-T2A-ScADH7mt | PWY1123 overexpressing Ilv2, Ilv5, Ilv3, ADH7 from S. cerevisiae and kivD from L. lactis | 0.1463 ± 0.0003 | 0.0368 ± 0.0015 | 30.4 ± 1.0 | 9.4 ± 0.3 | 0.114 ± 0.006 | This study |
PWY2343 | PWY2323 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3; YORW Δ22::PTDH3 –LlkivDmt-T2A-ScADH7mt | PWY2323 overexpressing Ilv2, Ilv5, Ilv3, ADH7 from S. cerevisiae and kivD from L. lactis | 0.1476 ± 0.0004 | 0.0431 ± 0.0004 | 43.3 ± 1.0 | 16.9 ± 1.9 | 0.226 ± 0.014 | This study |
PWY1153 | PWY1143 PHO13Δ | PWY1143 with PHO13 deleted | 0.1408 ± 0.0003 | 0.0513 ± 0.0002 | 33.9 ± 1.2 | 11.8 ± 0.8 | 0.134 ± 0.010 | This study |
PWY2353 | PWY2343 PHO13Δ | PWY2343 with PHO13 deleted | 0.1469 ± 0.0003 | 0.0532 ± 0.0004 | 43.0 ± 0.9 | 19.7 ± 2.4 | 0.172 ± 0.016 | This study |