Table 1 Strains generated in this study
From: Systematic improvement of isobutanol production from d-xylose in engineered Saccharomyces cerevisiae
Strain name | Genotype | Description | References |
|---|---|---|---|
BY4742 | Mat α; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0 | Laboratory strain | Baker Brachmann et al. (1998) |
PWY0013 | BY4742 ARS208::PTEF1-SsXKS; YMRWΔ15::PTEF1-HXTF79S | BY4742 overexpressing XKS from Scheffersomyces stipitis, HXT7_F79S from Saccharomyces cerevisiae | This study |
PWY1113 | PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-CtXYL2 | PWY0013 overexpressing XYL1 and XYL2 from Candida tropicalis | This study |
PWY1213 | PWY0013 ARS308::PTEF1-CsXYL1; ARS720::PTEF1-CtXYL2 | PWY0013 overexpressing XYL1 from Candida shehatae and XYL2 from C. tropicalis | This study |
PWY1313 | PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-CtXYL2 | PWY0013 overexpressing XYL1 from S. stipitis and XYL2 from C. tropicalis | This study |
PWY1413 | PWY0013 ARS308::PTEF1-SpXYL1.1; ARS720::PTEF1-CtXYL2 | PWY0013 overexpressing XYL1.1 from Spathaspora passalidarum and XYL2 from C. tropicalis | This study |
PWY1513 | PWY0013; ARS308::PTEF1-SpXYL1.2; ARS720::PTEF1-CtXYL2 | PWY0013 overexpressing XYL1.2 from S. passalidarum and XYL2 from C. tropicalis | This study |
PWY2113 | PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-SsXYL2 | PWY0013 overexpressing XYL1 from C. tropicalis and XYL2 from S. stipitis | This study |
PWY2213 | PWY0013 ARS308::PTEF1-CsXYL1; ARS720::PTEF1-SsXYL2 | PWY0013 overexpressing XYL1 from C. shehatae and XYL2 from S. stipitis | This study |
PWY2313 | PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-SsXYL2 | PWY0013 overexpressing XYL1 and XYL2 from S. stipitis | This study |
PWY2413 | PWY0013 ARS308::PTEF1-SpXYL1.1; ARS720::PTEF1-SsXYL2 | PWY0013 overexpressing XYL1.1 from S. passalidarum and XYL2 from S. stipitis | This study |
PWY2513 | PWY0013 ARS308::PTEF1-SpXYL1.2; ARS720::PTEF1-SsXYL2 | PWY0013 overexpressing XYL1.2 from S. passalidarum and XYL2 from S. stipitis | This study |
PWY3113 | PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-SpXYL2 | PWY0013 overexpressing XYL1 from C. tropicalis and XYL2 from S. passalidarum | This study |
PWY3313 | PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-SpXYL2 | PWY0013 overexpressing XYL1 from S. stipitis and XYL2 from S. passalidarum | This study |
PWY3413 | PWY0013 ARS308::PTEF1-SpXYL1.1; ARS720::PTEF1-SpXYL2 | PWY0013 overexpressing XYL1.1 and XYL2 from S. passalidarum | This study |
PWY3513 | PWY0013 ARS308::PTEF1-SpXYL1.2; ARS720::PTEF1-SpXYL2 | PWY0013 overexpressing XYL1.2 and XYL2 from S. passalidarum | This study |
PWY1123 | PWY1113 GRE3Δ | PWY1113 with GRE3 deleted | This study |
PWY1223 | PWY1213 GRE3Δ | PWY1213 with GRE3 deleted | This study |
PWY2323 | PWY2313 GRE3Δ | PWY2313 with GRE3 deleted | This study |
PWY3323 | PWY3313 GRE3Δ | PWY2313 with GRE3 deleted | This study |
PWY1133 | PWY1123 YORWΔ22::PTDH3 –LlkivDmt-T2A-ScADH7mt | PWY1123 overexpressing ADH7 from S. cerevisiae, and kivD from L. lactis | This study |
PWY1143 | PWY1133 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3 | PWY1133 overexpressing Ilv2, Ilv5, Ilv3 from S. cerevisiae | This study |
PWY1153 | PWY1143 PHO13Δ | PWY1143 with PHO13 deleted | This study |
PWY2333 | PWY2323 YORWΔ22::PTDH3 –LlkivDmt-T2A-ScADH7mt | PWY2323 overexpressing ADH7 from S. cerevisiae, and kivD from L. lactis | This study |
PWY2343 | PWY2333 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3 | PWY2333 overexpressing Ilv2, Ilv5, Ilv3 from S. cerevisiae | This study |
PWY2353 | PWY2343 PHO13Δ | PWY2343 with PHO13 deleted | This paper |