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Table 1 Strains generated in this study

From: Systematic improvement of isobutanol production from d-xylose in engineered Saccharomyces cerevisiae

Strain name Genotype Description References
BY4742 Mat α; his3Δ1; leu2Δ0; lys2Δ0; ura3Δ0 Laboratory strain Baker Brachmann et al. (1998)
PWY0013 BY4742 ARS208::PTEF1-SsXKS; YMRWΔ15::PTEF1-HXTF79S BY4742 overexpressing XKS from Scheffersomyces stipitis, HXT7_F79S from Saccharomyces cerevisiae This study
PWY1113 PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-CtXYL2 PWY0013 overexpressing XYL1 and XYL2 from Candida tropicalis This study
PWY1213 PWY0013 ARS308::PTEF1-CsXYL1; ARS720::PTEF1-CtXYL2 PWY0013 overexpressing XYL1 from Candida shehatae and XYL2 from C. tropicalis This study
PWY1313 PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-CtXYL2 PWY0013 overexpressing XYL1 from S. stipitis and XYL2 from C. tropicalis This study
PWY1413 PWY0013 ARS308::PTEF1-SpXYL1.1; ARS720::PTEF1-CtXYL2 PWY0013 overexpressing XYL1.1 from Spathaspora passalidarum and XYL2 from C. tropicalis This study
PWY1513 PWY0013; ARS308::PTEF1-SpXYL1.2; ARS720::PTEF1-CtXYL2 PWY0013 overexpressing XYL1.2 from S. passalidarum and XYL2 from C. tropicalis This study
PWY2113 PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-SsXYL2 PWY0013 overexpressing XYL1 from C. tropicalis and XYL2 from S. stipitis This study
PWY2213 PWY0013 ARS308::PTEF1-CsXYL1; ARS720::PTEF1-SsXYL2 PWY0013 overexpressing XYL1 from C. shehatae and XYL2 from S. stipitis This study
PWY2313 PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-SsXYL2 PWY0013 overexpressing XYL1 and XYL2 from S. stipitis This study
PWY2413 PWY0013 ARS308::PTEF1-SpXYL1.1; ARS720::PTEF1-SsXYL2 PWY0013 overexpressing XYL1.1 from S. passalidarum and XYL2 from S. stipitis This study
PWY2513 PWY0013 ARS308::PTEF1-SpXYL1.2; ARS720::PTEF1-SsXYL2 PWY0013 overexpressing XYL1.2 from S. passalidarum and XYL2 from S. stipitis This study
PWY3113 PWY0013 ARS308::PTEF1-CtXYL1; ARS720::PTEF1-SpXYL2 PWY0013 overexpressing XYL1 from C. tropicalis and XYL2 from S. passalidarum This study
PWY3313 PWY0013 ARS308::PTEF1-SsXYL1; ARS720::PTEF1-SpXYL2 PWY0013 overexpressing XYL1 from S. stipitis and XYL2 from S. passalidarum This study
PWY3413 PWY0013 ARS308::PTEF1-SpXYL1.1; ARS720::PTEF1-SpXYL2 PWY0013 overexpressing XYL1.1 and XYL2 from S. passalidarum This study
PWY3513 PWY0013 ARS308::PTEF1-SpXYL1.2; ARS720::PTEF1-SpXYL2 PWY0013 overexpressing XYL1.2 and XYL2 from S. passalidarum This study
PWY1123 PWY1113 GRE3Δ PWY1113 with GRE3 deleted This study
PWY1223 PWY1213 GRE3Δ PWY1213 with GRE3 deleted This study
PWY2323 PWY2313 GRE3Δ PWY2313 with GRE3 deleted This study
PWY3323 PWY3313 GRE3Δ PWY2313 with GRE3 deleted This study
PWY1133 PWY1123 YORWΔ22::PTDH3LlkivDmt-T2A-ScADH7mt PWY1123 overexpressing ADH7 from S. cerevisiae, and kivD from L. lactis This study
PWY1143 PWY1133 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3 PWY1133 overexpressing Ilv2, Ilv5, Ilv3 from S. cerevisiae This study
PWY1153 PWY1143 PHO13Δ PWY1143 with PHO13 deleted This study
PWY2333 PWY2323 YORWΔ22::PTDH3LlkivDmt-T2A-ScADH7mt PWY2323 overexpressing ADH7 from S. cerevisiae, and kivD from L. lactis This study
PWY2343 PWY2333 ARS1309::PTEF1-ScIlv2-T2A-ScIlv5-T2A-ScIlv3 PWY2333 overexpressing Ilv2, Ilv5, Ilv3 from S. cerevisiae This study
PWY2353 PWY2343 PHO13Δ PWY2343 with PHO13 deleted This paper