Fig. 4

Characterization of engineered strains with high-copy (2 µm) plasmid-based overexpression of individual pathway genes. Total isobutanol production (a), cell dry weight (b), xylose consumption (c), xylitol production (d), ethanol production (e), and glycerol production (f). Individual gene construct was placed in the pRSII426 plasmid and transform into PWY2353. The resulting strains were cultured in selective medium containing 2% xylose as the sole carbon source. Values are the mean of three biological replicates ± standard deviation (n = 3) after 3 and 6 days