Table 2 Oligonucleotides used

S9732N

5′-aaggccaacgaggccAGCAGATGCTGCAGC

Construction of pJOE9898.1 flanking sequences

S9733N

5′-aaggccagtctggccGATCAGCTTAATTGCGCTG

S9734N

5′-aaggcctaaatggccGACAGAAGCGGTGTGAC

S9735N

5′-aaggccttattggccGTCGGAGCAATGACGTTTA

S12531

5′-tacgGAATCCCTCACATCTGAAAT

Construction of pJOE9898.1 spacer

S12532

5′-aaacATTTCAGATGTGAGGGATTC

S12618

5′-GAAGCACTGATTCTATCACC

Colony PCR to prove the deletion by pJOE9898.1

S12619

5′-CATCCAGCCATTCAAATTGA

S9703N

5′-aaggccaacgaggccACTTGAAGGCCGTAACATT

Construction of pJOE9899.1, flanking sequences

S9704N

5′-aaggccagtctggccTGCTTAGCGGGCAGT

S9705N

5′-aaggcctaaatggccTGTTAATGATGCAAGGGCT

S9706N

5′-aaggccttattggccCGGCGTCCAATCGTTTTAC

S12533

5′-tacgAGAATTAGAAGATAAAATTG

Construction of pJOE9899.1, spacer

S12534

5′-aaacCAATTTTATCTTCTAATTCT

S12620

5′-CAGAGGACTGTACCATGAT

Colony PCR to prove the deletion by pJOE9899.1

S12619

5′-CATCCAGCCATTCAAATTGA

S9724N

5′-aaggccaacgaggccCAGGAAGCTGTTGAATCT

Construction of pJOE9918.1, flanking sequences

S9725N

5′-aaggccagtctggccGCCCTTCTTTTTCTACTCTA

S9726N

5′-aaggcctaaatggccGCTCATTTTCTTAAAAAGAATATC

S9727N

5′-aaggccttattggccGGCGATAAAGAATTCGAAG

S12529

5′-tacgGCAATCAGCTTCAGTCGAAA

Construction of pJOE9918.1, spacer

S12530

5′-aaacTTTCGACTGAAGCTGATTGC

S12660

5′-CGGCCTGTTGAATATTAAAC

Colony PCR to prove the deletion by pJOE9918.1

S12619

5′-CATCCAGCCATTCAAATTGA

S9707N

5′-aaggccaacgaggccCATGATGTTTGTCCCTAG

Construction of pJOE9957.1, flanking sequences

S9708N

5′-aaggccagtctggccGTTTCATACTGTGCTAAAGA

S9709N

5′-aaggcctaaatggccGTACCCTATAGTTATTTAAATCC

S9710N

5′-aaggccttattggccCCCAAATCGAACACGGTTCA

S12649

5′-tacgATGTCAGCAATACACACAAA

Construction of pJOE9957.1, spacer

S12650

5′-aaacTTTGTGTGTATTGCTGACAT

S12715

5′-GAATGCATCCACAGCAGG

Colony PCR to prove the deletion by pJOE9957.1

S12716

5′-GGCATAAATCACAGTGGCA

S9795

5′-aaggccaacgaggccGGGCTTGTCTTTATCGTG

Construction of pJOE9974.1, flanking sequences

S9796

5′-aaggccagtctggccGATGTGAAGACTGGAG

S9797

5′-aaggcctaaatggccGCCTGGCTTTGATTACGTG

S9798

5′-aaggccttattggccGTGCTCTCCGATAATATGC

S12673

5′-tacgGCTTATATCTATAAACATGA

Construction of pJOE9974.1, spacer

S12674

5′-aaacTCATGTTTATAGATATAAGC

S12740

5′-CGGTAAGTCCCGTCTAGC

Colony PCR to prove the deletion by pJOE9974.1

S12741

5′-GGGAAGCGTTCACAGTTTC

S12799

5′-aaggccagactggccTAAAAGTACAGTGCCGCTG

Amplification of Pgut-ganA

S12800

5′-aaggccatttaggccCCGAAAAGTGCCACCTG

S10494

5′-GACCTCAAAAAGGTCTTTA

DNA sequencing of inserts in pJOE8999.1

S11222

5′-CACGCATTGATTGAGTCAG