Table 1 Strains used in this study
Strain or plasmid | Genotype or relevant structure | Source or references |
|---|---|---|
E. coli | ||
JM109 | recA1, endA1, gyrA96, thi-1, hsdR17(r −K , m +k ), mcrA, supE44, gyrA96, relA1, λ−, Δ(lac-proAB), F’ (traD36, proAB + , lacIq, (ΔlacZ)M15) | Yanisch-Perron et al. (1985) |
B. subtilis | ||
Reg19 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS | Rahmer et al. (2015) |
JA-Bs34 | trpC2, ΔmanPA::ermC, PmtlAcomK-comS, Δ(spoIISA, spoIISB)::Pgut-ganA | This study |
JA-Bs35 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA) | This study |
JA-Bs36 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA), ΔnprB::Pgut-ganA | This study |
JA-Bs37 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA), ΔnprB::Pgut-ganA, Δ(ywrJ, cotB, cotH, cotG, ywrF, ywrE)::Pgut-ganA | This study |
JA-Bs40 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA), ΔnprB::Pgut-ganA, Δ(ywrJ, cotB, cotH, cotG, ywrF, ywrE)::Pgut-ganA, ΔamyE::Pgut-ganA | This study |