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Table 1 Strains used in this study

From: Multiple integration of the gene ganA into the Bacillus subtilis chromosome for enhanced β-galactosidase production using the CRISPR/Cas9 system

Strain or plasmid Genotype or relevant structure Source or references
E. coli
 JM109 recA1, endA1, gyrA96, thi-1, hsdR17(r K , m k + ), mcrA, supE44, gyrA96, relA1, λ, Δ(lac-proAB), F’ (traD36, proAB + , lacIq, (ΔlacZ)M15) Yanisch-Perron et al. (1985)
B. subtilis
 Reg19 trpC2, ΔmanPA::ermC, PmtlA-comK-comS Rahmer et al. (2015)
 JA-Bs34 trpC2, ΔmanPA::ermC, PmtlAcomK-comS, Δ(spoIISA, spoIISB)::Pgut-ganA This study
 JA-Bs35 trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA) This study
 JA-Bs36 trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA), ΔnprB::Pgut-ganA This study
 JA-Bs37 trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA), ΔnprB::Pgut-ganA, Δ(ywrJ, cotB, cotH, cotG, ywrF, ywrE)::Pgut-ganA This study
 JA-Bs40 trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δ(spoIIA, spoIIB)::Pgut-ganA, Δ(cotA, cotZ, cotY, cotX, cotW, cotC, yjcA, yjcK, yjcZ, spoVIB)::Pgut-ganA), ΔnprB::Pgut-ganA, Δ(ywrJ, cotB, cotH, cotG, ywrF, ywrE)::Pgut-ganA, ΔamyE::Pgut-ganA This study