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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Multiple integration of the gene ganA into the Bacillus subtilis chromosome for enhanced β-galactosidase production using the CRISPR/Cas9 system

Fig. 1

Restriction map of the expression vector pHWG1132 and the transcription and translation initiation region of ganA consisting of the gut promoter with the GutR binding site (arrows) and the − 35, − 10 and + 1 sequence (grey blocks). The sequence derived from gsiB is shown in italics, the ATG start codon in bold letters and restriction sites are underlined. The sequence between the BglII site and AflII site is derived from vector pJOE5751.1

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