Table 1 Components of the feed medium

Glucose

5 g/L glucose

Glutamine

8 mM glutamine

Hydrolysate

2 g/L yeast extract

Lipids^a

Cholesterol, tocopherol acetate, cold liver oil fatty acid methyl esters, Tween 80 and Pluronic F-68

Vitamins^b

Thiamine·HCl, riboflavin, d-calcium pantothenate

Pyridoxine·HCl, para-aminoben, nicotinic acid,

Zoic acid, i-inositol, biotin, choline chloride,

Vitamin B12, folic acid