Fig. 3
Cell growth profile and PCV2-VLPs production of the infected Sf9 cells from fed-batch and control batch cultures in shake-flask experiments. For the fed-batch cultures, sf9 cells were infected by the baculovirus Ac-orf2, at approximately 7 × 106 cells/mL at an MOI of 1. The feed was added when the cell density was 4.0 × 106 cells/mL and at the time of infection, as shown by the solid vertical arrows. Closed and open symbols represent the batch and fed-batch cultures, respectively; total cells (circle), cell viability (squares). The insert shows the volumetric PCV2-VLPs production expressed in mg/L for batch and fed-batch cultures after 96 hpi. Data represents the average of three independent experiments. Error bars represent standard deviation