The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization

AMB Express

Table 2 Comparison of the decolorization rates of indigo dye with four types of laccases

	Mediator (μM)	Opt. pH	Time (min)	Decolorization rate (%)
	Mediator (μM)	Opt. pH	Time (min)	wLcc9	rLcc9	PIE5	Tlac	Tlac^a
ABTS	200	7.0	180	71.9 ± 2.2	75.8 ± 0.2	90.9 ± 0.3	69.6 ± 1.3	84.3 ± 1.8
MS	120	7.5	50	70.2 ± 0.7	78.8 ± 0.9	87.1 ± 1.1	59.3 ± 1.8	84.3 ± 1.8

The data presented are the average values from triplicate parallel experiments and technical repeats of measurements (n = 9)
^aReaction was conducted at pH 5.0 and 60 °C according to the manufacturer’s instruction, using 200 μM indigo and 200 U L⁻¹ enzyme for 3 h, without addition of any mediator