Table 1 The optimum pH, temperature, redox potential, and kinetic constants of rLcc9, PIE5, and its mutants on two substrates
Proteins | Specific activity (U mg−1)a | Redox potential (mV)b | Guaiacol | 2,6-DMP | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
pH | Temp. (°C) | Km (M) | kcat (S−1) | kcat/Km (M−1 S−1) | pH | Temp. (°C) | Km (M) | kcat (S−1) | kcat/Km (M−1 S−1) | |||
rLcc9 | 315.3 | 505.7 | 6.5 | 70 | 0.9 × 10−4 | 22.8 | 2.5 × 105 | 6.5 | 60 | 2.3 × 10−3 | 69.4 | 3.0 × 104 |
PIE5 | 318.4 | 598.9 | 8.5 | 60 | 3.3 × 10−4 | 62.9 | 1.8 × 105 | 8.0 | 60 | 5.7 × 10−3 | 43.5 | 7.5 × 103 |
E116 K | 251.2 | 528.3 | 6.5 | 60 | 3.2 × 10−4 | 85.8 | 2.5 × 105 | 7.0 | 60 | 1.6 × 10−3 | 75.2 | 4.5 × 104 |
N229D | 477.9 | 575.4 | 8.0 | 60 | 3.7 × 10−4 | 87.3 | 2.3 × 105 | 8.0 | 60 | 2.6 × 10−3 | 66.2 | 2.5 × 104 |
I393T | 173.8 | 550.2 | 7.0 | 60 | 1.4 × 10−4 | 83.9 | 5.7 × 105 | 7.0 | 60 | 1.2 × 10−3 | 49.2 | 3.8 × 104 |
E116K/N229D | 253.2 | 582.7 | 8.0 | 60 | 3.4 × 10−4 | 75.4 | 2.1 × 105 | 8.0 | 60 | 3.0 × 10−3 | 38.0 | 1.2 × 104 |
E116K/I393T | 176.5 | 515.7 | 6.5 | 60 | 1.1 × 10−4 | 46.0 | 3.9 × 105 | 7.0 | 60 | 1.5 × 10−3 | 34.1 | 2.1 × 104 |
N229D/I393T | 363.3 | 556.5 | 8.0 | 60 | 3.4 × 10−4 | 82.5 | 2.3 × 105 | 8.0 | 60 | 2.9 × 10−3 | 38.8 | 1.3 × 104 |