Table 2 Primers used in this study (restriction sites are underlined)
Primer | Sequence (5′ → 3′) |
|---|---|
phaP1FAPstI | gatactgcagcgccaacctgatcgagcata |
phaP1RAXmaI | tatccccgggcttggccatggttctcaccc |
phaP1FBXmaI | gatacccgggaagaagtaagcgtctctcccg |
phaP1RBEcoRI | ctatgaattcaaggaactgctggtcatctc |
phaP1-FMfeI | ctagcaattgcatggccaagcagaccggtaa |
phaP1-RSmaI | atacccgggttacttcttggcgatgcgggc |
RT-16 s rRNA-F | ctgaacaaccagcgcatcga |
RT-16 s rRNA-r | aagtcctcgcgcttcacg |
RT-phaP1-F | gccgagatcgtccgttcct |
RT-phaP1-R | gcgaccttgaccagttcgg |
RT-phaP2-F | cccagtcctcgctggagaag |
RT-phaP2-R | gtctcgaagctcgccttggc |
RT-phaP3-F | agctcctgaagatctcctccga |
RT-phaP3-R | cctcacgggtcagctcctcg |
RT-phaP4-F | cctgtccgcacaagccca |
RT-phaP4-R | ccattcgcgcatgatggac |
RT-phaP5-F | gccaatccggagttcgtcc |
RT-phaP5-R | ccctgcatcagggtcatcca |
RT-phaP6-F | cgtcaaccagatgcagaccgtc |
RT-phaP6-R | cggagcagccttcggagc |