Table 1 Strains and plasmids used in this study
Bacteria | Characteristics | Reference |
|---|---|---|
Azospirillum brasilense Sp7 | Wild type strain | Tarrand et al. (1978) |
A. brasilense ΔphaP1 | A. brasilense Sp7 deleted in phaP1 gene. | This study |
A. brasilense PhaP1+++ | A. brasilense ΔphaP1 containing pMBA-12 | This study |
E. coli S17-1 | Smr, recA, thi, pro, hsdR-M+ RP4:2-Tc:Mu:Km Tn7, λpir | Simon et al. (1983) |
Plasmids | Characteristics | References |
|---|---|---|
pCR2.1TOPO | Source of Km resistance cassette | Invitrogen |
pGLO | Source of Ara promoter | BioRad |
pMMB206 | Expression vector | Morales et al. (1991) |
pSUP202 | Suicide vector | Roschanski and Strauch (2011) |
pSUPAB | pSUP202 containing upstream and downstream phaP1 flanking regions. | This study |
pSUPAKB | pSUPAB containing Km resistance cassette of pCR2.1TOPO | This study |
pMBA-2 | Expression vector pMMB206 derivative containing Ara promoter | This study |
pMBA-12 | pMBA-2 with phaP1 | This study |