Fig. 6

Analysis of the transcription of A. brasilense Sp7 phasin genes. RT-PCR assays of the internal region of putative phasin genes were conducted using cDNA of wild type (A. brasilense Sp7) and mutant (A. brasilense ΔphaP1) strains. RNA was extracted from cultures grown on minimal medium containing malic acid and ammonium chloride at C:N ratios of 30:1 and 90:1. Transcripts were visualized on a 3% agarose gel electrophoresis. a Transcription analyses of putative phasin genes of wild type and mutant strains grown at a C:N ratio of 30:1. For wild type: phaP1 (lane 1), phaP2 (lane 2), phaP3 (lane 3), phaP4 (lane 4), phaP5 (lane 5), phaP6 (lane 6). GeneRuler 1 Kb DNA ladder ThermoScientific (lane 7). For mutant strain: phaP2 (lane 8), phaP3 (lane 9), phaP4 (lane 10), phaP5 (lane 11), phaP6 (lane 12). 16S rRNA was used as positive control (lane 13). Negative control using cDNA of mutant strain with gene-specified primers for phaP1 transcript was done (lane 14). b Transcription analyses of putative phasin genes in wild type and mutant strains grown at a C:N ratio of 90:1. For wild type strain: phaP1 (lane 1), phaP2 (lane 2), phaP3 (lane 3), phaP4 (lane 4), phaP5 (lane 5), phaP6 (lane 6). GeneRuler 1 Kb DNA ladder ThermoScientific (lane 7). For mutant strain: phaP2 (lane 8), phaP3 (lane 9), phaP4 (lane 10), phaP5 (lane 11), phaP6 (lane 12). 16S rRNA was used as positive control (lane 13). Negative control using cDNA of mutant strain with gene-specified primers for phaP1 transcript was done (lane 14)