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AMB Express
Fig. 5 | AMB Express

Fig. 5

From: Phasin PhaP1 is involved in polyhydroxybutyrate granules morphology and in controlling early biopolymer accumulation in Azospirillum brasilense Sp7

Fig. 5

PHB/DCW content in A. brasilense strains. For PHB quantitation, flasks containing minimal medium supplemented with malic acid and ammonium chloride at C:N ratios of 30:1 (a and b), 60:1 (c and d), and 90:1 (e and f) were inoculated with the A. brasilense strains in flasks subjected to null (a, c and e) or low (b, d and f) oxygen transfer. Samples of cultures of A. brasilense were taken every 24 h for 96 h. Cells were harvested and dried, then PHB was extracted and quantified. PHB produced by A. brasilense Sp7 (WT), A. brasilense ΔphaP1 (ΔphaP1) and A. brasilense PhaP1+++ (PhaP1+++) are shown. Data are presented as mean ± standard deviation of three independent experiments. Letters in each graphic denote significant differences (p > 0.05) according to a one-way ANOVA test

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