Fig. 4

Transmission electron microscopy of ultra-thin sections of A. brasilense. Strains were grown on minimal medium containing malic acid and ammonium chloride at C:N ratios of 30:1 (a–c) and 90:1 (e–g). Strains were incubated at 32 °C and 110 rpm for 24 h. PHB granules of wild type (A. brasilense Sp7) (a and e), mutant (A. brasilense ΔphaP1) (b and f) and complemented (A. brasilense PhaP1⁺⁺⁺) (c and g) strains are shown as intracellular granules. Label Scale bar = 1 µm