Fig. 1

Phylogeny of A. brasilense Sp7 phasin proteins. a The amino acids sequences of phasin proteins of PHB-producing microorganisms were aligned to A. brasilense Sp7 proteins containing a phasin domain (PF09361). The conserved residues are highlighted. b A phylogenetic tree based on amino acid sequences of phasins was inferred using the Neighbor-Joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The analysis involved 18 amino acid sequences. All the positions containing gaps and missing data were eliminated. PhaP1AbSp7 to PhaP6AbSp7 denotes the A. brasilense Sp7 PhaP1 to PhaP6. PhaP1Reu to PhaP7Reu indicates the phasins PhaP1 to PhaP7 of R. eutropha. PhaIPpu and PhaFPpu are the phasins PhaI and PhaF, respectively, of P. putida. PhaP1Hse and PhaP2Hse are the H. seropedicae PhaP1 and PhaP2. PhaPAhy denotes the A. hydrophila PhaP