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Table 2 Primers used in this study

From: The significance of aspartate on NAD(H) biosynthesis and ABE fermentation in Clostridium acetobutylicum ATCC 824

Primers

Sequence (5′–3′)

Sources and references

Primers for gene amplification

 nadA-F

AGGAGGTTAGTTAGA GGATCC TCACATACACCCCTTATTTCCAAC

This work

 nadA-R

ACGACGGCCAGTGCC AAGCTT TCACCTTCCAAGTATTAACATATTT

This work

 nadB-F

AGGAGGTTAGTTAGA GGATCC ATGAATATTCAAACTGACGTATTAAT

This work

 nadB-R

ACGACGGCCAGTGCC AAGCTT TCAAATGTTGACCAATTCATTTTTC

This work

 nadC-F

AGGAGGTTAGTTAGA GGATCC ATGAATTGGTCAACATTTGATGAT

This work

 nadC-R

ACGACGGCCAGTGCC AAGCTT TTATTTTTCATTTCTTAAGTTTTTCATGC

This work

 NadC′-F

ACATCCCCCTTTCGC CAGCTG GAATCCATTTTGGGGGAAAAG

This work

 NadC′-R

CAGGCTTCTTATTTTTAT GCTAGC TTATTTTTCATTTCTTAAGTTTTTCATGC

This work

 pMTL-F

TGAAGTACATCACCGACGAGCAAG

This work

 pMTL-R

TGCTGCAAGGCGATTAAGTTGGGT

This work

 pMTL-R

CCTGTTGAACCATTAGCTAAGGA

This work

Primers for RT-PCR

 nadA(RT)-F

CAAAGACCTGAGGTGCAGGAA

This work

 nadA(RT)-R

GCCATTGGACAGCCAGCTT

This work

 nadB(RT)-F

CTTCAGGCGGTATAGGTGG

This work

 nadB(RT)-R

TCGCGAACGTCTATGTTATG

This work

 nadC(RT)-F

AAACGCTTAGAGGGCACAGG

This work

 nadC(RT)-R

TGCCGTCAGAAAGACCAAATC

This work

 CAC2679-F

GACATTACTTCAAACGAACCTG

Liao et al. (2018)

 CAC2679-R

CCCTTAGCCCATTTATTCCT

Liao et al. (2018)