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Table 1 Strains and plasmids used in this study

From: The significance of aspartate on NAD(H) biosynthesis and ABE fermentation in Clostridium acetobutylicum ATCC 824

Strains and plasmids Characteristics Sources and references
Strains
 C. acetobutylicum ATCC 824 Wild type ATCC
 E. coli DH5α DeoR, recA1, endA1, hsdR17 (rk, mk+) TIANGEN
 E. coli Top10 hsd R, mcr A, rec A1, end A1 TIANGEN
Plasmids
 pAN2 Φ3t I, p15A ori, TetR Heap et al. (2007)
 pMTL82151 ColE1 ori; CmR; pBP1 ori; TarJ Heap et al. (2009)
 pMTL-Pthl From pMTL82151; P-thl Liao et al. (2018)
 pMTL-Pthl thiCGE From pMTL82151; P-thl::thiC + thiG + thiE Liao et al. (2018)
 pMTL-Pthl-nadA From pMTL82151; P-thl::nadA This work
 pMTL-Pthl-nadB From pMTL82151; P-thl::nadB This work
 pMTL-Pthl-nadC From pMTL82151; P-thl::nadC This work
 pMTL-Pthl-thiCGE–nadC From pMTL82151; P-thl::thiC + thiG + thiE + nadC This work