Table 1 Strains and plasmids used in this study
Strains and plasmids | Characteristics | Sources and references |
|---|---|---|
Strains | ||
C. acetobutylicum ATCC 824 | Wild type | ATCC |
E. coli DH5α | DeoR, recA1, endA1, hsdR17 (rk−, mk+) | TIANGEN |
E. coli Top10 | hsd R, mcr A, rec A1, end A1 | TIANGEN |
Plasmids | ||
pAN2 | Φ3t I, p15A ori, TetR | Heap et al. (2007) |
pMTL82151 | ColE1 ori; CmR; pBP1 ori; TarJ | Heap et al. (2009) |
pMTL-Pthl | From pMTL82151; P-thl | Liao et al. (2018) |
pMTL-Pthl thiCGE | From pMTL82151; P-thl::thiC + thiG + thiE | Liao et al. (2018) |
pMTL-Pthl-nadA | From pMTL82151; P-thl::nadA | This work |
pMTL-Pthl-nadB | From pMTL82151; P-thl::nadB | This work |
pMTL-Pthl-nadC | From pMTL82151; P-thl::nadC | This work |
pMTL-Pthl-thiCGE–nadC | From pMTL82151; P-thl::thiC + thiG + thiE + nadC | This work |