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Table 1 The primers and gene sequences

From: Recombinant expressing angiopep-2 fused anti-VEGF single chain Fab (scFab) could cross blood–brain barrier and target glioma

 

5′ → 3′

Underline

ANG gene

ACCTTCTTTTACGGCGGAAGCAGGGGCAAGAGAAACAACTTCAAGACAGAGGAGTAC

 

PelB gene

ATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC

 

Linker 1

TCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGGT

 

Linker 2

GGAGGCGGTGGCTCAGGAGGTGGTGGGAGCGGTGGCGGCGGATCC

 

Light chain

F: GGAATTCCATATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGC

NdeI

R: CGGGATCCGGAGCACTCGGTGGGG

BamHI

Fd of heavy chain

F: GGAATTCTCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGC

TCTGAGGGTGGC

EcoRI

R1: CACCGCTTCCGCCTCCGCCGTGGGTCTTGTCGCAGGACTTG

Overlap

R2: CCGCTCGAGGTGGGTCTTGTCGCAGGACTTG

XhoI

ANG

F: CAAGTCCTGCGACAAGACCCACGGCGGAGGCGGAAGCGGTG

Overlap

R:GCTCTAGATTATTAGTGGTGGTGGTGGTGGTGATGGTGATGGTGATGATGGT

XbaI