5′ → 3′ | Underline | |
---|---|---|
ANG gene | ACCTTCTTTTACGGCGGAAGCAGGGGCAAGAGAAACAACTTCAAGACAGAGGAGTAC | |
PelB gene | ATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCC | |
Linker 1 | TCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGTGGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGGT | |
Linker 2 | GGAGGCGGTGGCTCAGGAGGTGGTGGGAGCGGTGGCGGCGGATCC | |
Light chain | F: GGAATTCCATATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGC | NdeI |
R: CGGGATCCGGAGCACTCGGTGGGG | BamHI | |
Fd of heavy chain | F: GGAATTCTCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGC TCTGAGGGTGGC | EcoRI |
R1: CACCGCTTCCGCCTCCGCCGTGGGTCTTGTCGCAGGACTTG | Overlap | |
R2: CCGCTCGAGGTGGGTCTTGTCGCAGGACTTG | XhoI | |
ANG | F: CAAGTCCTGCGACAAGACCCACGGCGGAGGCGGAAGCGGTG | Overlap |
R:GCTCTAGATTATTAGTGGTGGTGGTGGTGGTGATGGTGATGGTGATGATGGT | XbaI |