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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: Recombinant expressing angiopep-2 fused anti-VEGF single chain Fab (scFab) could cross blood–brain barrier and target glioma

Fig. 1

Construction of the scFab-ANG vector and identification of the activity of the fusion protein. a Illustration of vector pCold I-scFab-ANG. b Agarose gel electrophoresis of nucleic acid amplified by PCR, L1, L2, L3, L4, and L5 correspond to the gene segments of ANG, light chain, Fd domain of heavy chain, Fd-ANG and scFab, respectively. c SDS-PAGE gel electrophoresis of scFab-ANG and scFab proteins. Lane 1 is scFab-ANG, Lane 2 is scFab, Lane 3 is western blotting determination using total protein of hCMEC/D3 and LRP-1 molecular weight was about 85 kDa. d 4-parameter equation of indirect ELISA of scFab-ANG/scFab

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