Scientific validation of the antimicrobial and antiproliferative potential of Berberis aristata DC root bark, its phytoconstituents and their biosafety

AMB Express

Table 2 Qualitative detection and antimicrobial activity of Berberis aristata root bark

Phytoconstituents	Detected group	Stock solution (mg/mL)	Antimicrobial activity
*Alkaloids*		21.33	++^e
Mayer’s reagent test	+
Hager’s reagent test	+
Wagner reagent test	+
*Flavonoids*		68.33	+++^c
Shinoda test (magnesium turnings)	+^b
Zinc-hydrochloride reduction test	+
Lead acetate test	+
Ferric chloride reagent test	−^a
*Saponins*		NA	NA
Froth test	−
*Tannins*		73.5	−^f
Ferric chloride reagent test	−
Lead acetate test	+
*Cardiac glycosides*		NA	NA
Keller–Killiani test	−
*Terpenoids*
Triterpenes (Salkowski’s test)	+	10	+^d
Diterpenes (Copper acetate test)	+	87.42	+++^c
*Anthranol glycosides*		ND	ND
Borntrager’s test	+
*Phytosterols*		NA	NA
Libermann Burchard’s test	−
Salkowski’s test	−
*Coumarins*	+	ND	ND