Fig. 4

CLSM images of colonization of strain GJ-22-gfp on the leaves and root of tobacco. a–c and e–g were the results of foliar and root treatment for 24 h, 48 h and 72 h respectively. a Large numbers of fluorescence strains were observed on the surface of leaf without orderliness; b numerous of bacterial cells were adhered on the epidermal cell groove of tobacco leaves; c single cells formed aggregates were colonized the intercellular spaces; e numerous fluorescence cells were observed on the surfaces of primary roots; f long strings of green fluorescent cells were observed along the root epidermal cells; g single GJ-22-gfp cells were observed on root surface and occupied longitudinal intercellular spaces of roots surface. No cells were found in blank control leaf d and root h of tobacco. Magnification, ×20. And the experiment was repeated seven times