Fig. 4

Characterization of the N-glycans linked to the E2-CD154 proteins. a 10% SDS-PAGE under reducing conditions. MWM: Molecular weight marker; 1a and 1b: E2-CD154.1 and E2-CD154.2 proteins expressed by HEK293 cells in fermenter respectively; 2a and 2b: E2-CD154.1 and E2-CD154.2 after PNGase F treatment, respectively. The arrows indicate the putative non-glycosylated proteins. b Results of lectin specific assays to detect the quality of N-glycans bound to both E2-CD154 proteins, 1: E2-CD154.1; 2: E2-CD154.2. c NP-HPLC chromatograms of 2AB derivatives from E2-CD154N-glycan