Table 3 Comparisons between the molecular masses of G-hPRL of different origins, determined by MALDI–TOF-MS (Mr) and by N-glycoprofiling (MM)
From: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Host cell | MALDI–TOF-MS (Da) | N-Glycoprofilings (Da) | Difference MM/Mr (%) |
|---|---|---|---|
Human lactotrophsa | 24,903 | 24,736 | − 0.68 |
CHO cellsa | 24,970 | 25,016 | + 0.18 |
C127 cellsa | 25,139 | – | – |
HEK293 cellsb | 25,124 | 24,554 | − 2.27 |