Fig. 3
From: Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis
Separation of G-hPRL from NG-hPRL via RP-HPLC: a chromatogram showing the presence of four peaks: (1) eluted material without hPRL; (2) small fraction possibly due to a carbohydrate heterogeneity of HEK-G-hPRL; (3) main peak of HEK-G-hPRL; (4) NG-hPRL. b The same RP-HPLC chromatographic step, here expanded to show how the three fractions (#10–11–12) were collected for N-glycoprofiling determination