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Fig. 6 | AMB Express

Fig. 6

From: Molecular cloning and functional characterization of chalcone isomerase from Carthamus tinctorius

Fig. 6

Quantitative real time Expression levels of CtCHI gene and CHI activity in transgenic Arabidopsis lines. a Relative expression levels of CtCHI gene presented in four different transgenic lines in comparison with the wild type Arabidopsis. The data were normalized with 18s ribosomal RNA. b In vitro enzymatic activity of CHI using ELISA kit, RC9615, R&D Systems Inc., America). The color of the 3,3′,5,5′-tetramethylbenzidine was measured as absorbance (OD) using a spectrophotometer at 450 nm to calculate the concentration of CHI activity according to a standard regression curve. Data were presented as the mean ± SD. **P < 0.01 vs. the wild type group

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