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Table 1 Relative and specific activities of the three MtFae1a versions on FAE model substrates

From: Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

  M-Fae P-Fae E-Fae
Relative activity (%) Specific activity (µM/min/µg) Relative activity (%) Specific activity (µM/min/µg) Relative activity (%) Specific activity (µM/min/µg)
Methyl ferulate 47.5 ± 1.3 73.7 ± 2.0 64.9 ± 0.7 95.2 ± 1.1 37.6 ± 1.6 15.7 ± 0.7
Methyl caffeate 37.8 ± 4.0 58.7 ± 6.2 38.7 ± 1.9 56.8 ± 2.8 40.8 ± 3.2 17.1 ± 1.3
Methyl sinapate 19.9 ± 0.7 30.8 ± 1.1 24.8 ± 0.9 36.4 ± 1.3 Tracea Tracea
Methyl p-coumarate 100 ± 6.0 155 ± 9.3 100 ± 1.7 147 ± 2.5 100 ± 3.4 41.9 ± 1.4
  1. Data were obtained at pH 7.0 and 37 °C, using a continuous assay. Results are presented as the average of three experiments ± one standard deviation
  2. aTrace: trace activity observed (less than 0.4 µM/min/µg)