AMB Express

Table 1 Relative and specific activities of the three MtFae1a versions on FAE model substrates

From: Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

	M-Fae		P-Fae		E-Fae
	Relative activity (%)	Specific activity (µM/min/µg)	Relative activity (%)	Specific activity (µM/min/µg)	Relative activity (%)	Specific activity (µM/min/µg)
Methyl ferulate	47.5 ± 1.3	73.7 ± 2.0	64.9 ± 0.7	95.2 ± 1.1	37.6 ± 1.6	15.7 ± 0.7
Methyl caffeate	37.8 ± 4.0	58.7 ± 6.2	38.7 ± 1.9	56.8 ± 2.8	40.8 ± 3.2	17.1 ± 1.3
Methyl sinapate	19.9 ± 0.7	30.8 ± 1.1	24.8 ± 0.9	36.4 ± 1.3	Trace^a	Trace^a
Methyl p-coumarate	100 ± 6.0	155 ± 9.3	100 ± 1.7	147 ± 2.5	100 ± 3.4	41.9 ± 1.4

Data were obtained at pH 7.0 and 37 °C, using a continuous assay. Results are presented as the average of three experiments ± one standard deviation
^aTrace: trace activity observed (less than 0.4 µM/min/µg)

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