Fig. 3

Immunogold electron microscopy analysis of the binding of MAb 3E2 and SE50336 filament. a Bacteria cells were incubated with 3E2 MAb followed by reaction with colloidal gold-labelled goat anti-mouse IgG as described in “Materials and methods”. Colloidal gold particles (as the arrow showed) were visualized on the surface of filament demonstrate that 3E2 MAbs were able to recognize and bind to the filament of SE50336. b Negative control sample was incubated with same amount of PBS instead of 3E2 MAb