Fig. 1
From: A promising detection candidate for flagellated Salmonella spp.
Preparation and identification of recombinant rFliC′. a Analysis of fliC′ fragment by agarose gel electrophoresis. Lane M, molecular weight marker; lane 1, PCR-amplified fliC′ fragment. b Detection of the expression of rFliC′ in E. coli BL21 (DE3) by SDS-PAGE. Lane M, protein molecular weight marker; lane 1, pET22b (+) vector control; lane 2, BL21 (DE3) control; lane 3–7, cell lysate of pET-fliC after IPTG induction (0, 0.1, 0.4, 0.7 and 1.0 mmol/L); lane 8, Cell lysate supernatant of pET-fliC; lane 9, cell lysate precipitation of pET-fliC. c Analysis of the purity of the rFliC′ by SDS-PAGE. Lane M, protein molecular weight marker; lane 1, pET-fliC bacterial lysate; lane 2, purified rFliC′ protein. d Identification of rFliC′ by WB utilizing anti-His tag antibody. Lane M, protein molecular weight marker; lane 1, protein rFliC′