Rumen fermentation and microbial community composition influenced by live Enterococcus faecium supplementation

AMB Express

Table 4 Quantification of general bacteria, general fungi, methanogens, Fibrobacter succinogenes, and Ruminococcus flavefaciens by real-time PCR

Target genes	Treatments (log₁₀ copies number)				SEM	P-value
Target genes	Con	T1	T2	T3	SEM	Treatment	Linear	Quadratic
General bacteria	8.35	8.41	8.34	8.37	7.355	0.678	0.956	0.350
General anaerobic fungi	3.60^c	4.01^a	3.82^bc	3.97^ab	2.875	0.026	0.101	0.008
Methanogens	1.44^a	1.19^b	1.08^b	1.01^b	0.454	0.048	0.032	0.085
F. succinogenes	4.37^c	5.21^a	4.83^bc	4.93^b	3.958	0.010	0.028	0.005
R. flavefaciens	2.29^b	3.18^a	2.83^b	2.72^b	2.060	0.008	0.017	0.012

Con control (no addition), T1 0.1% E. faecium, T2 0.5% E. faecium, T3 1.0% E. faecium, SEM standard error of the mean
Different superscript letters indicate a statistically significant difference. P-value, calculated probability